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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Cryobiology. 2011 Apr 28;63(1):46–55. doi: 10.1016/j.cryobiol.2011.04.008

Figure 2. Assessment of Apoptosis and UPR Activation Following Hypothermic Exposure.

Figure 2

Figure 2

HCEC’s were placed at 4°C in either: Complete Media (CGM), HBSS with Ca++ and Mg++, ViaSpan, or OptiSol for 18 hours. Protein isolates were collected at 1, 4, 8 and 24 hours post-storage and western blot analysis was performed on these samples. (A) Analysis of apoptotic associated proteins revealed increased activation (increased caspase and PARP cleavage)in CGM and OptiSol stored samples that correlated with decreased viability. (B) Analysis of ER folding maintenance proteins demonstrated an increase in UPR specific protein activation in samples which were shown to have decreased viability. Specifically, there was a marked increase in ERO1-Lα and Bip levels for CGM and OptiSol stored samples as compared to controls as well as HBSS and ViaSpan stored counter-parts. Furthermore, a sustain level of PDI in addition to cleavage of calnexin was noted in these samples illustrating the further evidence of UPR specific activation.