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. 2012 Jun 14;10(6):1383–1390. doi: 10.3390/md10061383

Figure 3.

Figure 3

(A,B) Luciferase reporter assay. HepG2 cells, a hepatocarcinoma cell line, were transiently transfected with pSG5-PXR, pSG5-RXR, pCMV-βgalactosidase and p(CYP3A4)-TK-Luc vectors and then stimulated with (A) 10 µM rifaximin, solomonsterol A (2) or chalinulasterol (1) for 18 h, or (B) 10 µM rifaximin alone or in combination with 50 µM of compounds 1. Relative Luciferase Units were normalized with β-galactosidase Units (RLU/βgal). NT, not treated. *P<0.05 versus NT cells. Data are mean ± SE of three experiments. (C) Real-Time PCR of Cyp3A4. HepG2 cells were stimulated with 10 µM rifaximin, 2 or 1 for 18 h. Total RNA was extracted and the relative mRNA levels of PXR target gene Cyp3A4, was measured. Values were normalized relative to GAPDH mRNA and are expressed relative to those of untreated mice, which are arbitrarily set to 1. Analysis was carried out in triplicate and the experiment was repeated twice. NT, not treated. *P<0.05 versus NT cells.