Figure 2.
Scavenging reactive oxygen species restores autophagosome processing in myocardial ischemia-reperfusion injury, in vivo. A–F) Representative immunoblots (A) and quantitative analysis of LC3-II (B), p62 (C), LAMP2 (D), LAMP1 (E), and BECLIN-1 (F) expression in myocardial extracts from mice subjected to sham surgery or ischemia (30 minutes) followed by reperfusion (90 minutes), in the presence of MnTMPyP (6mg/kg i.p.) or saline control injected 1 hour prior to surgery. N=5–6/group. P values are by post-hoc test after one-way ANOVA.