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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: Circulation. 2012 May 16;125(25):3170–3181. doi: 10.1161/CIRCULATIONAHA.111.041814

Figure 6.

Figure 6

Hypoxia-reoxygenation-induced LAMP2 decline and BECLIN-1 upregulation are mediated via reactive oxygen species. A) Representative immunoblot (left) and quantitative analysis (right) of LAMP2 abundance in NRCMs subjected to hypoxia (6 hours) followed by reoxygenation (18 hours; Hyp/Reox) or normoxia (Nor) in presence of diluent (control) and MnTMPyP (50μmol/L); n=3/group. P values are by post-hoc test. B) Representative immunoblot (left) and quantitative analysis (right) of BECLIN-1 abundance in NRCMs treated as in A; n=4/group. P values are by post-hoc test. C) Representative immunofluorescence images (630X) depicting mCherry-GFP- LC3 expression in NCRMs treated as in A (Scale bars= 10μm); D) Quantitative analysis of autophagosomes (white bars), autolysosomes (black bars) and both (gray bars) in NRCMs treated as in A. ‘*’ indicates P< 0.05 for autophagosomes, and ‘#’ indicates P< 0.05 for total (autophagosomes+autolysosomes) as compared with respective normoxia control by post-hoc test. P values depicted are for comparisions between autophagosomes and autolysosomes by paired t-test within a group (n=15–20 nuclei/group).