Fig. 3. Trx1 overexpression altered the expression of TXNIP, AKAP12, VEGF, Bcl-2 and survivin during ischemic stress.
Representative Western blots show the expression of Trx1, TXNIP, AKAP12, VEGF, Bcl-2 and survivin, and their corresponding loading control-GAPDH. Western blot analysis revealed downregulation of AKAP12 (Fig. 3B) and TXNIP (Fig. 3C), whereas increased VEGF (Fig. 3D), Bcl-2 (Fig. 3E) and survivin (Fig. 3F) expression in TMI compared to the WMI. Numbers below the bands represent the average-fold change compared to WS from 3–5 independent experiments. Trx1 representative blot (Fig. 3A) is given for the validation of overexpression of Trx1 in transgenic animals. Abbreviations are as given in Fig. 1.