Table 2.
The most and least stable reference genes for each of the specific experimental conditions.
| Experiment sets | The three most stable genes | Optimal combination | The least stable gene |
|---|---|---|---|
| total | ACT, β-TUB1, β-TUB2 | V7/8 = 0.163 | 18S |
| tissues and developmental stage | ACT, β-TUB2, UBQ | ACT + β-TUB2 + UBQ (V3/4 = 0.168) | 18S |
| stresses | EF, α-TUB, UBQ | EF + α-TUB (V2/3 = 0.147) | GAPDH |
| tissues | ACT, β-TUB2, UBQ | (V4/5 = 0.186) | 18S |
| tissues (seedling) | EF, β-TUB2, ACT | EF + β-TUB2 (V2/3 = 0.087) | 18S |
| tissues (adult) | GAPDH, UBQ, β-TUB2 | GAPDH + UBQ (V2/3 = 0.113) | 18S |
| germination stage | EF, ACT, α-TUB | EF + ACT (V2/3 = 0.05) | GAPDH |