Table 1.

Nec-1 enhances shikonin-induced apoptosis in primary leukemia cells.

Patient No.	Sex	Age	Diagnosis	SHKconc. (μM)	Apoptotic rate (SHK)	Apoptotic rate (SHK + Nec-1)	p value (SHK vs. SHK + Nec-1)
1	M	74	AML	2.5	15.9 ± 2.5	24.3 ± 4.8	0.05669
2	F	49	AML	1.25	6.7 ± 3.7	20.6 ± 1.7	0.00398 **
3	M	24	AML	2.5	10.1 ± 4.5	18.8 ± 3.0	0.05162
4	M	28	CML	2.5	9.3 ± 2.8	15.2 ± 3.7	0.09042
5	F	54	CML	1.25	7.5 ± 3.2	15.4 ± 2.1	0.02372 *
6	M	33	CML	1.25	10.5 ± 2.4	19.9 ± 3.2	0.01594 *
7	F	35	CML	2.5	11.5 ± 1.9	18.5 ± 3.2	0.03051 *
8	M	43	CML	2.5	13.1 ± 4.6	18.6 ± 3.7	0.18326
9	M	53	AML	1.25	10.1 ± 1.0	13.3 ± 2.8	0.13721
10	F	18	AML	1.25	11.0 ± 3.6	24.8 ± 2.8	0.0065 **
11	M	55	CML	2.5	15.1 ±3.0	26.2 ± 4.0	0.01778 *
12	M	66	CML	2.5	13.8 ± 1.8	23.1 ± 2.8	0.00849 **
13	M	6	AML	1.25	23.3 ± 5.7	36.8 ± 7.2	0.0629
14	F	62	CML	1.25	35.8 ± 6.6	78.1 ± 7.7	0.00192 **

Note: Cells were incubated with 1.25 or 2.5 μM SHK for 12 h in the presence or absence of 60 μM Nec-1. Cells were harvested and assayed for Hoechst staining. Data represent means ± SD from triplicate of the same experiment. t-test

^*p < 0.05;

^**p < 0.01.