Table 1.
Patient No. | Sex | Age | Diagnosis | SHKconc. (μM) | Apoptotic rate (SHK) | Apoptotic rate (SHK + Nec-1) | p value (SHK vs. SHK + Nec-1) |
---|---|---|---|---|---|---|---|
1 | M | 74 | AML | 2.5 | 15.9 ± 2.5 | 24.3 ± 4.8 | 0.05669 |
2 | F | 49 | AML | 1.25 | 6.7 ± 3.7 | 20.6 ± 1.7 | 0.00398 ** |
3 | M | 24 | AML | 2.5 | 10.1 ± 4.5 | 18.8 ± 3.0 | 0.05162 |
4 | M | 28 | CML | 2.5 | 9.3 ± 2.8 | 15.2 ± 3.7 | 0.09042 |
5 | F | 54 | CML | 1.25 | 7.5 ± 3.2 | 15.4 ± 2.1 | 0.02372 * |
6 | M | 33 | CML | 1.25 | 10.5 ± 2.4 | 19.9 ± 3.2 | 0.01594 * |
7 | F | 35 | CML | 2.5 | 11.5 ± 1.9 | 18.5 ± 3.2 | 0.03051 * |
8 | M | 43 | CML | 2.5 | 13.1 ± 4.6 | 18.6 ± 3.7 | 0.18326 |
9 | M | 53 | AML | 1.25 | 10.1 ± 1.0 | 13.3 ± 2.8 | 0.13721 |
10 | F | 18 | AML | 1.25 | 11.0 ± 3.6 | 24.8 ± 2.8 | 0.0065 ** |
11 | M | 55 | CML | 2.5 | 15.1 ±3.0 | 26.2 ± 4.0 | 0.01778 * |
12 | M | 66 | CML | 2.5 | 13.8 ± 1.8 | 23.1 ± 2.8 | 0.00849 ** |
13 | M | 6 | AML | 1.25 | 23.3 ± 5.7 | 36.8 ± 7.2 | 0.0629 |
14 | F | 62 | CML | 1.25 | 35.8 ± 6.6 | 78.1 ± 7.7 | 0.00192 ** |
Note: Cells were incubated with 1.25 or 2.5 μM SHK for 12 h in the presence or absence of 60 μM Nec-1. Cells were harvested and assayed for Hoechst staining. Data represent means ± SD from triplicate of the same experiment. t-test
p < 0.05;
p < 0.01.