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Immunohistochemical studies for Toll-like receptor-4 (TLR4) and F4/80. (A) No staining was seen in the negative control group; (B) C3H/HeN mouse peritoneal macrophages were marked with F4/80 staining. Cells were approximately round; (C) The TLR4+ cells of C3H/HeN mice possessed round-ovoid morphology, expressed in the membrane without lipopolysaccharide (LPS) stimulation.
