Figure 2. Examples of immunohistochemical expression of Bax, Bcl-2, and p53nac in colorectal adenocarcinomas and adjacent benign epithelium. Examples of immunostaining of the adjacent benign colorectal epithelium are presented for Bax expression (Panel-A, x20), Bcl-2 expression (Panel-D, x20) and p53 nuclear accumulation (Panel-G, x20). Examples of immunostaining in the malignant regions of CRCs are shown for Bax expression (low level of expression in Panel-B, x40 and high level of expression Panel-C, x40), Bcl-2 expression (low level in Panel-E, x40 and high level in Panel-F, x40) and p53 nuclear accumulation (low level in Panel-H, x40 and high level in Panel-I, x40). Panel-C shows strong expression of Bax in stromal cells (thick arrow). Panels-D and E show strong Bcl-2 staining in lymphocytes (thick arrows) (internal positive controls) and weak staining in the basal colonic crypts (thin arrow in Panel-D) and in the malignant glands (thin arrow in Panel-E).
