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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Immunol. 2012 Jun 15;189(2):638–645. doi: 10.4049/jimmunol.1102579

Figure 8. Decreased avidity in d30 SLEC correlated with reduced levels of total and activated Lck and ZAP-70.

Figure 8

B8R-specific MPEC and SLEC present at d7 and d30 were analyzed for the expression of Lck (A) and ZAP-70 (B). Data represent the average MFI from 8–9 individual animals. For the analysis of phosphorylated molecules, CD8+CD44hiKLRG1+ or CD127+ cells were isolated by sorting. Cells were then incubated with tetramer to both identify the cells as well as initiate TCR signaling. Tetramer+ cells were analyzed following staining with phosphosrc-Tyr416 (C) or phospho-ZAP70 antibody (C). Data represent a total of 9 animals analyzed in 3 independent experiments. *, p≤0.05; **, p≤0.001.