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. Author manuscript; available in PMC: 2012 Jul 16.
Published in final edited form as: J Alzheimers Dis. 2010;20(4):1243–1253. doi: 10.3233/JAD-2010-100210

Figure 3. The predominant AβPP in the vitreous humor is the neuronal AβPP695 form.

Figure 3

AβPP in the aqueous (AQE) and vitreous (VIT) humors, retina (RET), and retinal pigment epithelium (RPE) were compared by Western blotting with the 6E10 antibody against the Aβ1–16 sequence (A) and R7 against the KPI domain absent in AβPP695 (B). Note the absence of the major sAPP band corresponding to AβPP695 in panel B with R7. R7 does detect a minor smaller band in the vitreous humor, but this protein migrates slightly faster than the major sAPPα - AβPP695 band detected by 6E10 in panel A and is relatively fainter than the 120-kDa sAPPα - AβPP751. To determine whether RPE cells express any AβPP695, we compared the AβPP bands in porcine RPE cells and the human ARPE-19 cell line with O443 to detect all AβPP forms and R7 to detect the KPI-containing AβPP751.

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