Figure 3.
Lumen widths can be controlled by varying channel geometry, incubation time, and hydrogel composition. (A) Lumens were generated through 4.5-mg/mL collagen I hydrogels after a 4-min incubation in straight microchannels of varying widths (no constrictions). (B) Lumens were generated through 4.5-mg/mL collagen I hydrogels after a 4-min incubation in microchannels with constrictions of varying widths adjacent to the inlet and outlet ports. (C) Lumens were generated through 4.5-mg/mL collagen I hydrogels after a 4-min incubation in microchannels with varying inlet port diameters. (D) Lumens were generated through 4.5-mg/mL collagen I hydrogels with varying incubation times. (E) Lumens were generated through collagen I hydrogels with varying concentrations after a 4-min incubation.