Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay

. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Methods Mol Biol. 2012;798:311–324. doi: 10.1007/978-1-61779-343-1_18

Reagents	Volume (μl)
10 × RT Buffer	2.0
20 × dNTP mix (100 mM)	0.8
10 × RT Random Primers	2.0
Multiscribe Reverse Transcriptase	1.0
Nuclease free water	4.2
Total	10.0