TABLE 1.
Quantification of LC20 mono- and diphosphorylation in Triton-skinned rat caudal arterial smooth muscle
LC20 phosphorylation levels were quantified by Phos-tag SDS-PAGE (see Fig. 4G, panel a) in tissues treated as described in the legend to Fig. 4, A–F. Values indicate the levels of unphosphorylated (0P), monophosphorylated (1P), and diphosphorylated LC20 (2P) under the conditions indicated.
| Conditions | % Total LC20 |
n | ||
|---|---|---|---|---|
| 0P | 1P | 2P | ||
| pCa4.5/pCa 4.5 | 52.1 ± 3.2 | 47.9 ± 3.2 | 0 | 4 |
| pCa 4.5/MC, pCa 4.5 | 0 | 40.3 ± 2.6 | 59.6 ± 2.7 | 5 |
| MC, pCa 4.5/MC, pCa 4.5 | 0 | 27.8 ± 3.8 | 71.0 ± 4.3 | 4 |
| pCa 9/pCa 9 | 100 | 0 | 0 | 3 |
| MC, pCa 9/MC, pCa 9 | 41.7 ± 3.1 | 30.6 ± 2.5 | 27.7 ± 2.5 | 5 |
| MC, pCa 9/MC, pCa 4.5 | 0 | 34.3 ± 4.3 | 64.4 ± 4.6 | 5 |