TABLE 3.
Thiophosphorylation of LC20 in Triton-skinned rat caudal arterial smooth muscle
Values represent percentage of total LC20 ± S.E. (n = 3). *, #, and ^ indicate values are not statistically significantly different from each other; 0P, unphosphorylated LC20; 1SP, monothiophosphorylated LC20; 1P, monophosphorylated LC20; 1SP1P, LC20 thiophosphorylated at one site and phosphorylated at the other; 2P, diphosphorylated LC20; RS, ATP regenerating system; MC, microcystin.
| Conditions | 0P | 1SP | 1P | 1SP1P | 2P |
|---|---|---|---|---|---|
| ATPγS, pCa 4.5, no RS | 6.0 ± 2.5* | 88.4 ± 4.8# | 0 | 0 | 5.6 ± 4.3^ |
| Then pCa 9, no RS | 11.1 ± 3.4* | 81.6 ± 3.9# | 0 | 0 | 7.2 ± 2.7^ |
| Then pCa 9, RS | 16.6 ± 4.7* | 80.0 ± 4.4# | 0 | 0 | 3.4 ± 1.9^ |
| Then MC, pCa 9, RS | 13.9 ± 3.2 | 54.7 ± 4.0 | 3.4 ± 2.1 | 14.0 ± 9.2 | 13.9 ± 8.6 |