FIGURE 7.

RP-HPLC analysis of the reactions of linoleic acid (left) and stearic acid (right) with cAOS/PhIO. The ¹⁴C-labeled fatty acids (50 μm) were reacted with cAOS (2 μm) and PhIO (50 μm) for 15 min at room temperature and extracted, and aliquots were analyzed by RP-HPLC (Waters Symmetry column, 25 × 0.46 cm) with on-line UV and radioactive detection. A solvent system of MeOH/H₂O/HAc was used in the proportions 90:10:0.01 for linoleic acid and 95:5:0.01 for stearic acid. The main peak from linoleic acid was detected at 205 nm in the UV (no 235 nm absorbance) and was identified as a 3:1 mixture of 12,13- to 9,10-linoleate monoepoxides by separation on SP-HPLC.