. 2012 May 16;287(29):24164–24173. doi: 10.1074/jbc.M112.341644

TABLE 1.

Plasmids generated in this study

Plasmid	Resistance^a	Source plasmid	Genotype
panaTic22	Amp^R	pET21b^b
panaOMP85-POTRA^c	Amp^R	Topo-TrcHis2^d
pJT13	Sp^R/Sm^R	pCSEL24^e	pCSEL24 (P_alr0114/GFP)
pJT14	Sp^R/Sm^R	pRL271^f	pRL271 (orf_alr0114 bp 1–404/Sp^R/Sm^R/423–825)
pALH1	Nm^R	pRL271	pRL271 sacB::Nm^R
pALH2	Nm^R	pALH1	pALH1 (P_petJ/alr0114)
pALH3	Nm^R	pALH1	pALH1 (P_petJ/alr0114 D213R)
pALH4	Nm^R	pALH1	pALH1 (P_petJ/alr0114 I219R)
pALH5	Nm^R	pALH1	pALH1 (P_petJ/alr0114 F136G)
pALH6	Nm^R	pALH1	pALH1 (P_petJ/ssalr0114-atTic22)

^a Amp^R, ampicilin; Sp, spectinomycin; Sm, streptomycin; Nm, neomycin.

^b Novagen.

^c Ref. 16.

^d Invitrogen.

^e Ref. 56.

^f Ref. 57.