TABLE 2.
Used and generated strains
Sp (spectinomycin), Sm (streptomycin), Nm (neomycin) AFS (Anabaena sp., mutant created in Frankfurt by a member of the Schleiff group), AFS-I (AFS with an insertion in the gene specified), and AFS-PDGF (promoter downstream GFP fusion).
| Strain | Resistance | Genotype | Relevant properties | References |
|---|---|---|---|---|
| AFS-I-tic22 | SpR/SmR | alr0114::pJT14 | Gene interruption | This study |
| AFS-Ι-alr0075 | SpR/SmR | alr0075::SpR/SmR | Gene interruption | Ref. 23 |
| AFS-Ι-alr2269 | SpR/SmR | alr2269::SpR/SmR | Gene interruption | Ref. 23 |
| AFS-Ι-alr2270 | SpR/SmR | alr2270::SpR/SmR | Gene interruption | Ref. 23 |
| AFS-I-alr4893 | SpR/SmR | alr4893::SpR/SmR | Gene interruption | Ref. 23 |
| AFS-I-alr2887 | SpR/SmR | alr2887::SpR/SmR | Gene interruption | Ref. 22 |
| AFS-PDGF-tic22 | SpR/SmR | nucA region::pJT13 | Promoter-GFP fusion | This study |
| AFS-I-tic22 petJ/anatic22 | SpR/SmR/NmR | alr0114::pJT14 pALH2 | Complementation | This study |
| AFS-I-tic22 petJ/D213R | SpR/SmR/NmR | alr0114::pJT14 pALH3 | Complementation | This study |
| AFS-I-tic22 petJ/I219R | SpR/SmR/NmR | alr0114::pJT14 pALH4 | Complementation | This study |
| AFS-I-tic22 petJ/F136G | SpR/SmR/NmR | alr0114::pJT14 pALH5 | Complementation | This study |
| AFS-I-tic22 petJ/atTic22 | SpR/SmR/NmR | alr0114::pJT14 pALH6 | Complementation | This study |