FIGURE 2.
Deglycosylation of gp120 with endo- and exoglycosidases. The indicated glycosidases were used to treat the following gp120s under mild conditions. A, 293T cell-produced gp120 that contains both complex and oligomannose (i.e. heterogeneous) N-glycans; B, oligomannose-enriched gp120kif from kifunensine-treated 293T cells (i.e. Man9GlcNAc2 N-glycans); C, oligomannose-enriched gp120GnTI−/− from 293S GnTI−/− cells (i.e. Man6Man5–9GlcNAc2 N-glycans). The enzyme-treated gp120 variants were analyzed by reducing SDS-PAGE and Western blot. The positions of the molecular mass marker proteins and the approximate apparent molecular mass of the enzyme-treated gp120s are indicated; D, glycosidase treatment of gp120GnTl−/− under mild conditions. The gp120s were analyzed by SDS-PAGE analysis and Western blotting.