FIGURE 4.
Time- and pH-dependence of Endo H-mediated deglycosylation of KNH1144 SOSIP.664Gntl−/− trimers. The trimers were treated with Endo H at 37 °C in buffers with varying pH (0.05 m glycine, pH 2.7; 0.075 m sodium citrate, pH 4.0; 0.075 m sodium citrate, pH 5.0; 0.1 m BisTris, pH 6.0; 0.1 m HEPES, pH 7.0; 0.1 m CAPS, pH 9.5) and for varying times, before analysis by BN-PAGE. The species associated with each band are indicated with arrows (d, deglycosylated; partially deglyc., partially deglycosylated).