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Protease sensitivity of deglycosylated gp140 trimers. SOSIP.681GnTl−/− trimers were either Endo H-treated or mock-treated, and then exposed to different serine proteases at a range of concentrations (0.001–1 mg/ml): A, proteinase K; B, trypsin; C, chymotrypsin. The treated proteins were analyzed by SDS-PAGE and silver staining.
