TABLE 1.

SEC-UV/MALS/RI analysis of Endo H-treated and untreated SOSIP.664_GnTl−/−

	UV extinction coefficient A2800.1%a	Estimatedb (dn/dc)glycoprotein d	Expected MMproteinc of monomer	MMproteinfrom SEC-UV/MALS/RIe,f	Calculated stoichiometry	MMglycan from SEC-UV/MALS/RI	MMglycoprotein from SEC-UV/MALS/RI	Hydrodynamic radius, rHf
	ml mg−1cm−1	ml/g	g/mol			g/mol		nm
Untreated SOSIP.664GnTl−/−	1,649	0.168	74,332.6	220,600	2.97	173,500	394,200	12.3
Endo H-treated SOSIP.664GnTl−/−	1,649	0.179	74,332.6	223,500	3.01	36,500	260,000	9.9

^a Extinction coefficient at 280 nm measured in water.

^b Refractive index increment value.

^c Molar mass.

^d From the basic principles of molar mass determination by light scattering, it follows that any ± 1% error in the dn/dc value will propagate as a corresponding ± 1% error in the calculated molar mass (59).

^e For SEC-UV/MALS/RI analysis of glycoproteins, a typical error is ± 10%. The source of uncertainty predominantly arises from the amount of sample analyzed and the stability of the system (59).

^f Errors propagated in the various calculations performed by Astra V range between 0.2 and 50%.