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Binding of H10-03-6 variants to protein A and CD16a. Protein A-coated Biacore chips were used to capture H10-03-6 glycovariants. After a washing step, recombinant soluble CD16a was added to the protein A-captured Fcabs followed by an injection of plain buffer. Association and dissociation phases are shown in the sensograms. Signal of an uncoated reference cell was subtracted from all measurements.
