Knockdown of Mmp1a-PAR1 decreases invasion of LLC1 lung cancer cells.
A and B, Mmp1a mRNA (A) and Mmp1a protein expression (B) following stable, lentiviral knockdown in LLC1 cells with Mmp1a-targeted shRNA (shMmp1a-1 or -2) versus shLuc (luciferase) control. C and D, Matrigel (C) and collagen (D) invasion of Mmp1a knockdown LLC1 cells. E, LLC1 cell invasion through collagen following stable transduction with a PAR1-targeted shRNA (shPar1) versus shLuc control (left panel), with FACS analysis of PAR1 surface expression (right panel) using S19 FITC-PAR1 Ab versus isotype control (gray). All data represent means ± S.E. of three experiments. *, p < 0.05; **, p < 0.005. WB, Western blot.