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Actin-activated MgATPase activities of sk S1 (A) and myosin V HMM (B). Reactions were performed at 23 °C. Data were fitted to the Michaelis-Menten equation. Error bars indicate S.D. Three to seven measurements were made at each actin concentration, except for 35 and 40 μm actin in the assay using sk S1. At those concentrations, measurements were made only once due to the limited availability of the recombinant actin.
