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. 2012 May 29;287(29):24378–24386. doi: 10.1074/jbc.M112.371807

FIGURE 1.

FIGURE 1.

Metabolic effects of acute 4d HFD feeding. A and B, body (A) and epididymal fat weights (B) of wild type 6-week-old C57BL/6 male mice under HFD (60%) for 4 days, compared with age-matched male mice on LFD (13% fat). n = 12–15 mice with three repeats. C, histology section of adipose tissue. D, measurement of diameters of 450 adipocytes from 3–4 mice on 4d HFD (gray) and age-matched LFD (white). E, Whisker plot of adipocyte diameters. F, serum insulin and glucose levels in WT mice (following a 6 h fast for insulin and 16 h fast for glucose) on 60% HFD for 4 days or 8 weeks, compared with age-matched mice on LFD (13% fat). n = 8–10 mice each, 2 repeats. G and H, GTT and ITT of WT mice on LFD or 4d HFD. n = 15 each cohort, three repeats. Note that glucose intolerance develops within days of HFD feeding. Mice were placed on HFD at the age of 6 weeks. Values represent mean ± S.E. *, p < 0.05; **, p < 0.01; and ***, p < 0.005.