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4d HFD feeding increases IL-4 levels in WAT via NKT cells. A, Q-PCR analysis of IL-4 and IFN-γ mRNA levels following 4d HFD feeding in adipose tissue of WT and CD1d−/− mice (n = 12–15 mice each group, three repeats). B, Western blot analyses of p-Y STAT proteins in IL-4-treated purified primary adipocytes and SVC of adipose tissue from WT mice (two repeats). Values represent mean ± S.E. **, p < 0.01.
