FIGURE 6.

Effect of Src-specific inhibitor PP2 or Src siRNA on UGT2B15 expressed in COS-1 cells. A, 70 h after transfection with pSVL-UGT2B15, cells were treated with different concentrations of PP2 before harvesting to assay for DHT and 5α-androstane-3α,17β-diol (ADT DIOL). Western blot analysis was carried out with anti-UGT-1168, anti-Src, and anti-β-actin antibodies as described under “Experimental Procedures.” B, effect of Src siRNA on UGT2B15. Twenty-four h after transfection with pSVL-UGT2B15, cells were treated with different concentrations of either Src siRNA or non-target siRNA for 44 h as described under “Experimental Procedures.”