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Accessory Sec-independent transport of GspB736FLAG and selected variants in E. coli. The amino acid sequence of the H and C regions of the signal peptide, along with the AST domain of GspB is indicated. The GspB736FLAG variants were expressed constitutively from pVA891 (26, 27), and were detected by Western blotting with anti-FLAG monoclonal antibodies. Lane 1, periplasmic proteins; lanes 2–8, whole-cell lysates.
