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. 2012 May 31;287(29):24438–24447. doi: 10.1074/jbc.M112.378059

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Transport of a GspB_736FLAG chimera by SecA2 chimeras in S. gordonii. A, diagram of SecA2 and the SecA2 chimeras. The putative functional domains are demarcated as described previously (19). NBD, nucleotide binding domain; PPXD, preprotein binding domain. A thrombin cleavage site in the S. gordonii SecA2 is indicated (Th). B, diagram of the GspB_736FLAG/spAST preprotein chimera, and secretion of the GspB_736FLAG and GspB_736FLAG/spAST from S. gordonii. GspB_736FLAG and GspB_736FLAG/spAST were detected by Western blotting with anti-FLAG antibodies. Lane 1, PS1226 with pM99secA2/asc; lane 2, PS2812 with pM99secA2/asc; lane 3, PS1226 with pM99secA2CH3; lane 4, PS2812 with pM99secA2CH3.