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. 2012 May 22;287(29):24473–24482. doi: 10.1074/jbc.M112.356113

FIGURE 3.

FIGURE 3.

Detection of the intermediate pVP2-452 protein species in IBDV-infected QM7 cells. IBDV-infected QM7 cultures were harvested at 8, 24, 30, and 48 hpi, and the corresponding extracts were analyzed by Western blot analysis. The major bands corresponding to the precursor pVP2 and mature VP2 polypeptides is indicated on the right. The arrow indicates the position of the intermediate pVP2 protein with the same mobility as pVP2-452. Bands corresponding to mature VP2 (441 residues) and pVP2-452 proteins generated by expression of truncated polyprotein genes are indicated (left).