FIGURE 2.

COUP-TFII binds to the proximal renin promoter in its native context. Cross-linked nuclear extracts isolated from As4.1 (top) or Calu-6 cells (bottom) were used in ChIP with COUP-TFII antibody (COUP-TFII Ab) followed by isolation of the precipitated DNA. DNA isolated from non-precipitated samples was used as positive control (Input). The input and the COUP-TFII antibody-precipitated samples were subjected to PCR with a primer pair amplifying a proximal renin promoter fragment containing the COUP-TFII recognition sequence proxDR (promoter) or with a primer pair amplifying a non-related exon sequence that served as a negative control (exon).