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. 2012 May 29;287(29):24483–24491. doi: 10.1074/jbc.M111.329474

FIGURE 5.

FIGURE 5.

COUP-TFII and the proxDR sequence are necessary for the cAMP-induced expression of the renin gene mediated by a CRE site in the proximal promoter. A, COUP-TFII is necessary for the cAMP-induced renin gene transcription in Calu-6 cells. COUP-TFII was knocked down with sequence-specific siRNA (siCOUP-TFII). Control cells were transfected with nontargeting siRNA (siControl). Calu-6 cells were transfected with the 258-bp human proximal promoter construct proxDR containing the COUP-TFII binding site, and the effect of cAMP agonists on its transcriptional activity was tested. B, Calu-6 cells were transfected with the proxDR construct, with construct proxDR-CREmut carrying the mutated cAMP target site CRE, with construct proxDR-CNREmut carrying the mutated cAMP target site CNRE, or with construct proxDRdel in which proxDR was deleted and the effect of cAMP agonists on the transcriptional activity was tested. The data shown are means ± S.E. (error bars). *, p < 0.05.