FIGURE 5.

Interaction of vimentin and phosphorylated MAPK in RBL-CCR1 cells stimulated via FcϵRI and CCR1. After sensitization with anti-DNP-IgE, RBL-CCR1 cells were stimulated with 10 ng/ml DNP-HSA and/or various concentrations of rCCL3 for 5 min. A, after the stimulation, soluble forms of vimentin were extracted and analyzed by immunoblotting using anti-vimentin Abs. B, the cells were incubated with β,β′-iminodipropionitrile (IDPN (+)) to promote aggregation of vimentin intermediate filaments for the last 1 h of sensitization. Soluble forms of vimentin in the cells were extracted and subjected to immunoprecipitation using anti-vimentin mAb and analyzed by immunoblotting using anti-phosphorylated ERK1/2 mAb, anti-phosphorylated p38 kinase mAb, or anti-vimentin Abs. The data are representative of three independent experiments.