FIGURE 2.

Two TLR9 variants show reduced NF-κB activation in response to CpG-ODN. A and B, HEK293T cells were transfected with TLR9 WT, 111X, F667L, R863Q, or R892W and assayed for NF-κB activity with (black) or without (gray) CpG-ODN stimulation by dual luciferase assay (A) or immunoblotted for TLR9 and β-tubulin to confirm equivalent expression levels (B). C, HEK293T cells were transfected with the indicated amounts of TLR9 WT (80, 40, or 20 ng) in the presence of TLR9 111X or TLR9 R892W (20, 60, or 80 ng). The cells were CpG-ODN stimulated (black) or unstimulated (gray). pCI, empty vector transfected. p values of < 0.05 and <0.01 were denoted as * and **, respectively. One representative of three independent experiments is shown. In A and C, triplicate points ± S.D. are shown. RLU, relative light unit(s); IB, immunoblot.