FIGURE 6.

Kindlin-2 and talin head domain can associate simultaneously with the integrin β₃ CT. A, kindlin-2, talin-H, or their mixture, at the same individual concentrations (700 nm), was injected over the sensor chips coated with full-length β₃ CT, and their association properties were recorded. B, the β₃ CT coated sensor chip was first saturated with a high concentration (2.8 μm) of talin-H. Once the binding reached the maximal level, kindlin-2 (700 nm) was injected. Increasing concentrations of talin-H were injected over sensor chips coated with full-length β₃ CT with (C) or without (D) kindlin-2 in running buffer.