FIGURE 2.

Suppression of the increase in fluorescence after bleaching in the outer (A) and inner (B) segments of isolated rod photoreceptors deficient in Rdh8. Lack of Rdh12 does not affect the change in fluorescence in either compartment. Fluorescence was excited with 360-nm light and emission collected >420 nm. This fluorescence signal was due to an unknown mix of retinol and retinal but was dominated by retinol in wild type and Rdh12^−/− and by retinal in Rdh8^−/− and Rdh8^−/−;Rdh12^−/− (see Fig. 4). The change in fluorescence is plotted as a function of time after bleaching for 129/Sv wild-type (○, n = 6), Rdh12-deficient (▴, n = 11), and Rdh8-deficient (▾, n = 12) mice and mice deficient in both Rdh8 and Rdh12 (●, n = 14). Error bars, S.E. All of the experiments were done at 37 °C.