FIGURE 3.

The emission properties of the fluorescence that appears in mouse rod photoreceptor outer and inner segments after bleaching are consistent with those of retinoids and are different from those of NADH. Ratios of the emission at 528 nm over that at 457 nm (Fem-528/Fem-457)) for the outer and inner segment fluorescence changes for different mouse strains, wild type (wt, n = 7 cells), Rdh12^−/− (n = 8), Rdh8^−/− (n = 9), and Rdh8^−/−;Rdh12^−/− (n = 9), were measured 30 min after bleaching of dark-adapted intact cells. The ratios for retinal (RAL, n = 8) and retinol (ROL, n = 9) were measured 15 min after adding 50 μm of exogenous retinoid to intact Rdh8^−/−;Rdh12^−/− cells. The ratio for NADH was measured with a solution of 200 μm NADH (n = 4). Error bars, S.E. All of the experiments were done at 37 °C.