FIGURE 6.

The inner segments (B and D) Rdh12-deficient mice have a lower capacity to reduce exogenously added retinal compared with wild-type mice; their outer segments (A and C) have similar capacity as wild type. A, fluorescence increase in the outer segments of rod photoreceptors from 129/Sv (○, n = 7) and Rdh12-deficient (▴, n = 9) mice, at different times after bleaching. All-trans-retinal (5 μm) was added at 30 min after bleaching. B, fluorescence in the inner segment of the cells shown in A. C, fluorescence increase in the outer segments of rod photoreceptors from 129/Sv (○, n = 9) and Rdh12-deficient (▴, n = 4) mice, at different times after bleaching. All-trans-retinal (1 μm) was added at 30 min after bleaching. D, fluorescence in the inner segments of the cells shown in C. The fluorescence values have been normalized to that immediately before the addition of retinal. Error bars, S.E. All of the experiments were done at 37 °C.