FIGURE 6.

Effects of inhibiting iPLA₂β or 12/15-LO on TNF-α-induced TNF-α or IL-6 mRNA expression in cultured VSMC. Rat aortic VSMC (A–C, E, and F) and mouse aortic VSMC (D) were pretreated with various inhibitors for 30 min and/or stimulated with TNF-α (10 ng/ml) for 5 min (A) or 1 h (B–F). A, effects of TNF-α stimulation on iPLA₂-specific activity. B, effects of BEL (10 μm) or luteolin (50 μm) on TNF-α-induced TNF-α mRNA expression. C, effects of iPLA₂β antisense or sense oligonucleotides on TNF-α-induced TNF-α mRNA expression. D, effects of genetic deletion of iPLA₂β on TNF-α-induced TNF-α mRNA expression. E, effects of BEL (10 μm) or luteolin (50 μm) on TNF-α-induced IL-6 mRNA expression. F, effects of iPLA₂β antisense or sense oligonucleotides on TNF-α-induced IL-6 mRNA expression. Results are expressed as mean ± S.E. from at least three independent experiments. *, p < 0.05; ***, p < 0.001 versus basal. †††, p < 0.001 versus vehicle (Veh, Me₂SO₂) in B and E or sense/TNF-α in C and F. NS, no significance.