FIGURE 1.

Allosteric Hsp90 inhibitor reduces TDP43 levels. A, Western blot of HeLa cell lysates treated with celastrol, 17-AAG, or vehicle control for 24 h. B, quantitation of Western blot by densitometry showing percentage levels of full-length (FL) and cleaved (C) TDP-43 following celastrol treatment (dark gray bars) or 17-AAG treatment (light gray bars) normalized to GAPDH relative to vehicle treated ± S.D. *, p < 0.05 by Student's t test. C, equal amounts of protein from HeLa cell lysates immunoprecipitated (IP) with rabbit anti-TDP-43 or control rabbit IgG antibodies. Cell lysates (inputs) and immunoprecipitates were analyzed by Western blotting for Hsp90, Cdc37, TDP-43, and GAPDH antibodies.