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U87 cells and GSDCs express SMa and Flk-1 but not endothelial cell markers. A, cell lysates of U87 cells, GSDCs, MDA-MB-231 cells, and HMVECs were analyzed by immunoblotting against Flk-1, SMa, VE-cad, CD31, Tie1, Tie2, N-cad, and actin. MDA-MB-231 cells were used as a negative control. B, these cells were also used for immunocytochemistry staining of SMa, Flk-1, and VE-cad. DAPI is nuclear staining. Bars, 50 μm.
