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The structure of parental probe is provided at the top. EMSA results of 5 µM Tt-RpoE1 protein binding with single-stranded (ssTop, ssBot.) or fork-junction structure promoter DNA(T+1/B−10); the vertical line indicates the terminal base-pair on the strands used in fork-junction probe. Free probe (T+1/B−10) was loaded as a negative control. The arrows indicate complexes formed by Tt-RpoE1 and the different probes.
