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. 2012 Jul 16;7(7):e40885. doi: 10.1371/journal.pone.0040885

Figure 4. The effect of putative anti-σ factor Tt-TolB on the activity of Tt-RpoE1.

Figure 4

(A). The effect of Tt-TolB on the interaction between Tt-RpoE1 and fork-junction structure promoter DNA (T+1/B−10). The indicated proteins were added (+) in an EMSA reaction at a concentration of 5 µM. The solid arrow indicates the supershifted complex formed by Tt-RpoE1, Tt-TolB, and the promoter, and the open arrow indicates the complex formed by Tt-RpoE1 and the promoter. (B). The effect of Tt-TolB on in vitro transcription of Tt-RpoE1. Lane 1. Tt-TolB was added into the transcription system at the same time with Tt-RpoE1. Lane 2. Tt-TolB was added into the transcription system after Tt-RpoE1, E.coli core RNAP and promoter DNA being incubated (see materials and methods). Lane C, the in vitro transcription system without Tt-TolB. The solid arrow indicates the products of transcription.