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. 2012 Jul 16;7(7):e40885. doi: 10.1371/journal.pone.0040885

Figure 6. The effect of scanning substitutions in the −35 and −10 regions on the activity of Tt-RpoE1.

Figure 6

(A & B). EMSA results of substitutions in the −35 (A) and −10 (B) regions. The D−5/−10 or D−5/−12 fork-junction structure of promoter DNA was used as the parental probe respectively. The substitutions were made both on the top and opposite positions of the bottom strands in the duplex part as indicated. (C). The effect of a subset of substitutions in the −10 region on in vitro transcription of Tt-RpoE1.