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. 2012 Jul 16;7(7):e40885. doi: 10.1371/journal.pone.0040885

Figure 7. The effects of single amino acid substitution in Region 2.4 of Tt-RpoE1 on the interaction between Tt-RpoE1 and promoter DNA.

Figure 7

(A). Alignment of the amino acid sequence of Regions 2.4 in group IV ECF σ factors. The numbers at each end of the sequence indicate the amino acid position. The substitutions in Tt-RpoE1 used in this study are shown. The asterisk indicates the conserved amino acids of the melting residues of σ70. Species abbreviations and GenBank accession numbers of their proteins are as follows: Thermoanaerobacter tengcongensis (T.tc), Tt-RpoE1 (NP_622011.1); Bacillus subtilis (B.sub), SigX (NP_390191.2), SigW (NP_388054.1); Pseudomonas aeruginosa (P.aer), PvdS (NP_251116.1); Myxococcus Xanthus (M.xan), CarQ (YP_632266.1). The recognition between D66 and −12G is indicated by arrow. (B). The effects of single amino acid substitution in Region 2.4 of Tt-RpoE1 on the interaction between Tt-RpoE1 and wild-type (D−5/−12) or mutated promoter DNA. Tt-RpoE1 D66A uniquely suppressed single nucleotide changes at position −12G.