Table 2. Partial oligonucleotides used in this study.
| Names | Sequences(5′ to 3′)* | Purposes |
| P1 | GCGAATTCAGCTTTATTGAATTTTATGAG | P1/P2:BD-Tt-RpoE1 |
| P2 | TCGGATCCTCATCCCTCCAAACATTT | |
| P3 | TCCTCGAGTCATCCCTCCAAACATTT | P1/P3: p28Tt-RpoE1 |
| P4 | ATGAATTCCGGCGAGTTTCAGCAAGT | P4/P2: pPTt-RopE1-T |
| P5 | AGGAATTCGACGAAAAGAGAATAGAG | P5/P6: AD-Tt-TolB |
| P6 | TTGGATCCGTATTAAAACCTGCCCTT | |
| P7 | CTCTCGAGTATCTTTTTCCATCTGTT | P5/P7: AD-Tt-TolBN |
| P8 | AGGAATTCCAAGATAATTTAATAACA | P8/P6: AD-Tt-TolBC |
| P9 | ACGAATTCATGGACGAAAAGAGAATAGA | P9/P10: p28Tt-TolB |
| P10 | CTCTCGAGGTATTAAAACCTGCCCTT | P8/P10: p28Tt-TolB-C |
| P11 | TGGAATTCAGTACTAGCTCTTTGATTTT | P11/P12: AD-Permase |
| P12 | TGGGATCCGGCCTGCCTTAGTTGATG | |
| P13 | CGGAATTCGTGGAAATAGAGCTTAAAAA | P13/P14: AD-PtsB |
| P14 | CGGGATCCTCACTTTATCATCTCCTTTA | P4/P15 for transcription template |
| P15 | CTTTGAGTTTGATTTTAC | |
| P16 | ACCGGTACACATCGTCAAAGTTTT | For primer extension |
| P17 | CCGAAATACTGTGACAGCCTATTACAGAATGAGGA | P17/18: D66A of p28Tt-RpoE1 |
| P18 | TCCTCATTCTGTAATAGGCTGTCACAGTATTTCGG | |
| P39 | TTTTGTGTAGATTTTTGTCTATAAAGGTGGGAGGAG | P39/40:mutation at −13C of transcription template |
| P40 | CTCCTCCCACCTTTATAGACAAAAATCTACACAAAA | |
| P41 | TTTGTGTAGATTTTCTTCTATAAAGGTGGGAGGAGT | P41/42:mutation at −12G of transcription template |
| P42 | ACTCCTCCCACCTTTATAGAAGAAAATCTACACAAA | |
| P43 | TTGTGTAGATTTTCGCCTATAAAGGTGGGAGGAGTC | P43/44:mutation at −11T of transcription template |
| P44 | GACTCCTCCCACCTTTATAGGCGAAAATCTACACAA | |
| P45 | TGTGTAGATTTTCGTTTATAAAGGTGGGAGGAGTCA | P45/46:mutation at −10C of transcription template |
| P46 | TGACTCCTCCCACCTTTATAAACGAAAATCTACACA | |
| P47 | GTGTAGATTTTCGTCGATAAAGGTGGGAGGAGTCAA | P47/48:mutation at −9T of transcription template |
| P48 | TTGACTCCTCCCACCTTTATCGACGAAAATCTACAC |
*
Restriction sites are underlined.