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30 µM of unmodified DsrC (A) and DsrC mutant proteins carrying a Cys-Ser mutation in DsrC-Cys100 (B) or DsrC-Cys111 (C) were incubated with 2 µM IscS and 2 mM cysteine or with 2 mM sulfide. Note that for the unmodified DsrC results are shown for the double charged molecule.
