Figure 8. IP-10p is able to inhibit angiogenesis.

A) C57BL/6J mice were subcutaneously implanted with 750 µl of Matrigel containing VEGF (23.3 µM), IP-10 (1.2 µM) and/or, of IP-10p (41 µM). Ten days post inoculation; the Matrigel plug was removed and stained with Masson Trichrome to visualize endothelial infiltration. Vessels were quantified by counting vessel infiltration in to the plug on day 10. B) To verify that IP-10p is able to inhibit regression of newly formed vessel in vivo GFR- Matrigel supplemented with VEGF (10.4 µM) was injected into both the left and right side groin of mice. After 10 days the left side Matrigel plug was removed (control, n = 6) to show vessel invasion. The remaining right side Matrigel plug in the mice was injected with either IP-10 (1.2 µM) and/or, IP-10p (41 µM) at days 10 and 12. Day 17, the Matrigel plugs were removed and stained with Masson’s Trichrome. C) To validate the invasion of endothelial cells plugs were stained with CD31. IP-10p treated plugs showed a significant regression of vessels compared to the saline controls. D) Vasculature is a marker of vessel maturation Desmin to validate mature vessels. Images shown represent N = 8 Original magnifications, 10X and/or 40X.