Table 1. Primer sequences for CHART-PCR and ChIP assay.
Primer position | Sequences (5′ - 3′) | CNS |
−57/+166 | F : GTTTGCCAGCCTTTGGAGC | Pro |
R : CGAGGCGGATCATGGCTCAC | ||
+976/+1178 | F : CTATCGTTAGAGGTGGCAGCTGTATAG | CNS 1a |
R : CATACAGAGAAAGACAGGGCTGGAAC | ||
+2014/+2405 | F : GACATTGACCCACATCTGCATTTCTCAG | CNS 1b |
R : GCTACCTCTTTCGGGGAACTGTTTTG | ||
+2900/+3068 | F : CTGAGGTGGAGGAGCAGGGAGTATC | CNS 1c |
R : TCTGCTCTTCAAGGAGGGCGAGAATCC | ||
+6397/+6663 | F: GCTTCTAAATTGCTACTCTCTTACCTGGCAGC | CNS 2a |
R: CCAGAGAAATACAAGTGCCCAGTCATTCTTTG | ||
+7628/+7767 | F : CTGTTCTTCAGCATCCACCAGGCTTC | CNS 2b |
R : CATTTGGACCGAGACGCTGGCCTTG | ||
+14049/+14335 | F : GTGGGCAAAAGCTGAGGTACCCAGAAGG | CNS 3 |
R : CAGGCGTAGAAAAGCAACCTGATGCCAG | ||
+28700/+29122 | F : CTGACCTGACCTGATGATACCCA | 3′ UTR |
R : GGGCATGCCTTATAGAACCAAGG |
Region encompassed by the analyzed CNSs are:
Promoter;−57/+166, CNS 1; +850/+3334, CNS 2; +5755/+9165, CNS 3; +14013/+14613, 3′ UTR; +28700/+29243.